E-ISSN 2602-4837
May zonula occludens proteins regulate the pathogenesis of allergic rhinitis? [Tr-ENT]
Tr-ENT. 2019; 29(2): 100-106 | DOI: 10.5606/Tr-ENT.2019.64936

May zonula occludens proteins regulate the pathogenesis of allergic rhinitis?

Özge Yılmaz1, Esra Toprak Kanık1, Ercan Pınar2, Ahmet Türkeli1, Elgin Türköz Uluer3, Sevinç İnan3, Hasan Yüksel1
1Department of Pediatric Allergy and Pulmonology, Celal Bayar University Medical Faculty, Manisa, Turkey
2Department of Otorhinolaryngology, Atatürk Training and Research Hospital, Izmir, Turkey
3Department of Histology and Embryology, Celal Bayar University Medical Faculty, Manisa, Turkey

INTRODUCTION: This study aims to investigate the expression pattern of zonula occludens (ZO) proteins, namely occludin, claudin-1, tricellulin, junctional adhesion molecules (JAM), and ZO-1, -2, and -3 in nasal mucosal biopsies of individuals with and without allergic sensitization.
METHODS: Between August 2011 and August 2012, a total of 69 patients (38 males, 31 females; mean age 28.0 years, range, 18 to 61 years) who underwent surgery for nasal septum deviation were included in this cross-sectional study. All patients underwent skin prick test with environmental allergen mixtures. Cup forceps biopsy samples were obtained from the inferior turbinate during septoplasty. These samples were stained immunohistochemically for occludin, claudin-1, tricellulin, JAM, and ZOs. Staining intensity was graded semi-quantitatively using the H-Score.
RESULTS: Of all patients, 14 were atopic. Occludin, claudin-1, and JAM scores were significantly lower in the mucosal samples from atopic patients, compared to the non-atopic patients (median 142.5 vs. 288, 153 vs. 296, and 156 vs. 312, respectively; p<0.001 for all). The ZO-1, -2, and -3 proteins were significantly lower in atopic patients (p<0.001 for all). The tricellulin, located at the intersection of three epithelial cells, was not significantly different between the two groups (208.5 vs. 195, respectively; p=0.686).
DISCUSSION AND CONCLUSION: Expression of the structural proteins of ZO decreases in the upper airways of asymptomatic atopic patients. These findings indicate that ZO may be an important determinant of atopic sensitization and, therefore, may be a potential target in the treatment of allergic rhinitis.

Keywords: Allergic rhinitis, atopy, tricellulin, zonula occludens.

Özge Yılmaz, Esra Toprak Kanık, Ercan Pınar, Ahmet Türkeli, Elgin Türköz Uluer, Sevinç İnan, Hasan Yüksel. May zonula occludens proteins regulate the pathogenesis of allergic rhinitis?. Tr-ENT. 2019; 29(2): 100-106

Corresponding Author: Özge Yılmaz, Türkiye
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