E-ISSN 2602-4837
[Tr-ENT]
Tr-ENT. 2018; 28(1): 34-39 | DOI: 10.5606/Tr-ENT.2018.22556

Effects of methotrexate in a toluene diisocyanate-induced allergic rhinitis rat model

Sibel Alicura Tokgöz1, Gökçe Şimşek2, Erkan Vuralkan3, Ömer Bayır1, Fulya Köybaşıoğlu4, Murat Çalışkan5, Ömer Beşaltı5, İstemihan Akın1
1Department of Otorhinolaryngology, Dışkapı Yıldırım Beyazıt Training and Research Hospital, Ankara, Turkey
2Department of Otorhinolaryngology, Medical Faculty of Kırıkkale University, Kırıkkale, Turkey
3Department of Otorhinolaryngology, Trabzon Numune Training and Research Hospital, Trabzon, Turkey
4Department of Pathology, Dışkapı Yıldırım Beyazıt Training and Research Hospital, Ankara, Turkey
5Department of Surgery, Medical Veterinary Faculty of Ankara University, Ankara, Turkey

INTRODUCTION: This study aims to investigate the therapeutic effect of low-dose topical administration of methotrexate (MTX) in a toluene diisocyanate (TDI)-induced allergic rhinitis rat model.
METHODS: The experiments were performed on 18 healthy male Wistar albino rats weighing between 250-270 g. Rats were divided into four study groups: control group (n=5), sham group (n=3), steroid group (n=5), and MTX group (n=5). After the drugs were administered, multiple biopsies were taken bilaterally from the nasal mucosal areas and evaluated histologically for fibrosis, loss of cilia and goblet cells, edema, squamous cell metaplasia, and vascular proliferation.
RESULTS: Fibrosis, loss of cilia cells, edema, and vascular proliferation were significantly lower in both MTX and steroid groups than in the control group (p<0.05). There were no statistically significant differences with respect to histopathological parameters between the steroid group and the MTX group (p>0.05). There were no statistically significant differences with respect to loss of goblet cells and squamous cell metaplasia among the studied groups (p>0.05).
DISCUSSION AND CONCLUSION: Methotrexate may be an alternative or adjuvant therapeutic agent in allergic rhinitis.

Keywords: Allergic rhinitis, methotrexate, toluene diisocyanate.

Sibel Alicura Tokgöz, Gökçe Şimşek, Erkan Vuralkan, Ömer Bayır, Fulya Köybaşıoğlu, Murat Çalışkan, Ömer Beşaltı, İstemihan Akın. Effects of methotrexate in a toluene diisocyanate-induced allergic rhinitis rat model. Tr-ENT. 2018; 28(1): 34-39

Sorumlu Yazar: Sibel Alicura Tokgöz, Türkiye
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